Molecular Biology Platform

Laboratory:

• Molecular Genetics Laboratory
• Tissue and Tumor Bank

Activities: Nucleic acid extraction and qualitative / quantitative evaluation

• Amplification of DNA sequences;
• Quantitative / semi-quantitative techniques for nucleic acid analysis by Real-time PCR;
•  Assessment of methylation status and loss of heterozygous state (LOH);
•  Molecular analysis of gene expression by reverse-transcription techniques;
•  Evaluation of gene expression by Microarray techniques;
•  Analysis of gene mutations / polymorphisms through:

1. techniques using restriction enzymes - PCR-RFLP technique (Polymerase Chain reaction - Restriction Fragments Lenght Polymorphisms)
2. SSCP technique (Single Strand Confirmation Polymorphism)
3.  ARMS-PCR technique - PCR (Amplification-refractory mutation system - PCR)
4. MLPA technique (Multiplex Ligation-Dependent Probe Amplification)
5. DNA sequencing using SANGER methods
6. Next Generation Sequencing techniques (NGS)<img alt="" data-cke-saved-src="http://cedmog.ro/files/photos/124_thumbnail.jpg" src="http://cedmog.ro/files/photos/124_thumbnail.jpg" "="">